Quantitative evaluation of gonadal proteins in male and female oysters (Crassostrea virginica ) using an immunological technique

Simple item page
dc.acquisition-srcDownloaded from-Aquatic Sciences and Fisheries Abstractsen_US
dc.call-noen_US
dc.contract-noen_US
dc.contributor.authorChoi KSen_US
dc.contributor.authorLewis DHen_US
dc.contributor.authorPowell ENen_US
dc.contributor.otheren_US
dc.date.accessioned2010-02-15T17:14:26Z
dc.date.available2010-02-15T17:14:26Z
dc.date.issued1989 1990 Apr 1en_US
dc.degreeen_US
dc.description-en_US
dc.description-otheren_US
dc.description.abstractA polyclonal antibody has been produced against egg protein and sperm protein of the American oyster for obtaining a weight-based gonadal index. The gonadal index was expressed in the form: mg gonadal protein/g wet wt oyster tissue. The polyclonal antibody against oyster sperm protein was produced by stripping ripe sperm into phosphate-buffered saline. Sperm was collected, homogenized and injected into an Albino New Zealand rabbit. Rabbit anti-oyster sperm IgG (10 mu g/ml) detected 0.2 to 6.0 mu g of sperm protein. To assess the usefulness of this method, 50 female oysters were collected from Deer Island, Galveston Bay, Texas from June through October, 1988. During that period, monthly gonadal index (for females) varied between 107 mg/g (June) and 22 mg/g (October). The maximum gonadal index recorded was recorded in July, 365 mg/g, and the minimum in October, 0.012 mg/g. Maximal indices in July indicate that the gonad may account for as much as 36% of the body weight during the spawning seasonen_US
dc.description.urihttp://gbic.tamug.edu/request.htmen_US
dc.historyen_US
dc.identifier.urihttp://hdl.handle.net/1969.3/23077
dc.latitudeen_US
dc.locationen_US
dc.longitudeen_US
dc.notes1990 Annu. Meet. of the National Shellfisheries Association, Williamsburg, VA (USA)0730-8000Summary onlyEnglishJournal Article; Conference; SummaryMarineen_US
dc.placeen_US
dc.publisheren_US
dc.relation.ispartofseries50639.00en_US
dc.relation.urien_US
dc.scaleen_US
dc.seriesJournal of Shellfish Research [J. SHELLFISH RES.]en_US
dc.subjectantibodiesen_US
dc.subjectCrassostreaen_US
dc.subjectCrassostrea virginicaen_US
dc.subjectEvaluationen_US
dc.subjectGalveston Bayen_US
dc.subjectImmunologyen_US
dc.subjectMethodologyen_US
dc.subjectO 8050 CONFERENCESen_US
dc.subjectOyster cultureen_US
dc.subjectOystersen_US
dc.subjectProteinsen_US
dc.subjectQ1 01264 Reproduction and developmenten_US
dc.subjectQ1 01583 Shellfish cultureen_US
dc.subjectQ3 01583 Shellfish cultureen_US
dc.subjectShellfishen_US
dc.subjectspawningen_US
dc.subjectspermen_US
dc.subjectTexasen_US
dc.subjectUSAen_US
dc.titleQuantitative evaluation of gonadal proteins in male and female oysters (Crassostrea virginica ) using an immunological techniqueen_US
dc.typeCONFen_US
dc.universityen_US
dc.vol-issue()en_US

Files

Collections

Galveston Bay Bibliography
GBIC Abstracts (full text not available through Jack K. Williams Library)