A polyclonal antibody developed from Perkinsus marinus hypnospores fails to cross react with other life stages of P. marinus in oyster (Crassostrea virginica) tissues.

Simple item page
dc.acquisition-srcen_US
dc.call-noAcc# 4704en_US
dc.contract-noen_US
dc.contributor.authorChoi, K.S.en_US
dc.contributor.authorLewis, D.H.en_US
dc.contributor.authorPowell, E.N.en_US
dc.contributor.authorFrelier, P.F.en_US
dc.contributor.authorRay, S.M.en_US
dc.contributor.otherJournal of Shellfish Researchen_US
dc.date.accessioned2010-02-15T17:10:21Z
dc.date.available2010-02-15T17:10:21Z
dc.date.issued1991en_US
dc.degreeen_US
dc.descriptionp. 411-415en_US
dc.description-otheren_US
dc.description.abstractPolyclonal antiserum was produced from Perkinsus marinus hypnospores harvested from oyster tissue cultivated in fluid thioglycollate medium. The specificity of the antiserum for hypnospores was tested using indirect sandwich ELISA with alkaline phosphatase - conjugated goat anti-rabbit IgG and indirect immunofluorescence. As little as 20 ng. of hypnospore protein could be detected by ELISA. Immunofluorescence assays suggested that the antigenic material was a component of the spore cell wall. Cross-reactivity of the antiserum to other life stages of P. marinus present in oyster tissues could not be demonstrated by ELISA or immunofluorescence indicating that a substantial change in the antigenic properties of the cell wall occurs during spore formation. Hypnospore formation was also induced by placing P. marinus - infected oyster tissues into an anaerobic chamber rather than fluid thioglycollate. Spores were positively identified by ELISA, however little spore enlargement occurred suggesting that the triggering mechanism for spore formation is not the same as that for enlargement.en_US
dc.description.urihttp://gbic.tamug.edu/request.htmen_US
dc.geo-codeGulf of Mexicoen_US
dc.geo-codeGalveston Bayen_US
dc.historyen_US
dc.identifier.urihttp://hdl.handle.net/1969.3/22480
dc.latitudeen_US
dc.locationGalveston Bay Collection; TAMUG periodical collectionen_US
dc.longitudeen_US
dc.notesResearch conducted under grant from U.S. Department of Commerce, National Oceanic and Atmospheric Administration Sea Grant College Program, #NA89AA-D-SG139.en_US
dc.placeen_US
dc.publisheren_US
dc.relation.ispartofseries4704.00en_US
dc.relation.urien_US
dc.scaleen_US
dc.seriesen_US
dc.subjectoystersen_US
dc.subjectCrassostrea virginicaen_US
dc.subjectPerkinsus marinusen_US
dc.subjectparasitismen_US
dc.subjectantibodiesen_US
dc.titleA polyclonal antibody developed from Perkinsus marinus hypnospores fails to cross react with other life stages of P. marinus in oyster (Crassostrea virginica) tissues.en_US
dc.typeArticleen_US
dc.universityen_US
dc.vol-issue10(2)en_US

Files

Collections

Galveston Bay Bibliography
GBIC Materials Available at Jack K. Williams Library