Analysis of biogenic thiols in natural water samples by high-performance liquid chromatographic separation and fluorescence detection with ammonium 7-fluorobenzo-2-oxa-1,3-diazole-4-sulfonate (SBD-F)

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dc.acquisition-srcDownloaded from-Web of Scienceen_US
dc.call-noen_US
dc.contract-noen_US
dc.contributor.authorTang DGen_US
dc.contributor.authorWen LSen_US
dc.contributor.authorSantschi PHen_US
dc.contributor.otherAnalytica Chimica Actaen_US
dc.date.accessioned2010-02-15T17:17:13Z
dc.date.available2010-02-15T17:17:13Z
dc.date.issued2000 Mar 9en_US
dc.degreeen_US
dc.description299-307en_US
dc.description-otheren_US
dc.description.abstractA sensitive and reliable reversed-phase high-performance liquid chromatography (HPLC) method has been developed to analyze naturally occurring low-molecular weight thiols using pre-column derivatization with ammonium 7-fluorobenzo-2-oxa-1,3-diazole-4-sulfonate (SBD-F) and fluorescence detection in a gradient elution. The derivatization procedure and separation conditions were optimized by careful control of the reaction conditions in terms of time, temperature, pH and reducing agent. A linear calibration in the nM range was obtained for all the standard compounds, allowing measurement of thiols in natural water samples. Results show that the reducing agent tri-n-butylphosphine (TBP) not only acts as an anti-oxidant/disulfide cleaving agent, but also enables the SBD-F to react with thiols complexed by soft metals. This is the first study reported using the SBD-F labeling technique to measure phytochelatin concentrations. The methodology has been successfully applied to estuarine water samples from Galveston Bay. (C) 2000 Elsevier Science B.V. All rights reserveden_US
dc.description.urihttp://gbic.tamug.edu/request.htmen_US
dc.historyen_US
dc.identifier.urihttp://hdl.handle.net/1969.3/23460
dc.latitudeen_US
dc.locationen_US
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dc.notesTimes Cited: 13ArticleEnglishTang, D. GTexas A&M Univ, Dept Oceanog, 5007 Ave U, Galveston, TX 77551 USACited References Count: 16293EDPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSAMSTERDAMen_US
dc.placeen_US
dc.publisheren_US
dc.relation.ispartofseries51107.00en_US
dc.relation.urien_US
dc.scaleen_US
dc.seriesen_US
dc.subjectwater analysisen_US
dc.subjectthiolsen_US
dc.subjectphytochelatinen_US
dc.subjectsoft metalsen_US
dc.subjectFLUORESCENCE DETECTIONen_US
dc.subjectPLASMAen_US
dc.subjectcopperen_US
dc.subjectALGAEen_US
dc.titleAnalysis of biogenic thiols in natural water samples by high-performance liquid chromatographic separation and fluorescence detection with ammonium 7-fluorobenzo-2-oxa-1,3-diazole-4-sulfonate (SBD-F)en_US
dc.typeJournalen_US
dc.universityen_US
dc.vol-issue408(1-2)en_US

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