Isoelectric shifts in ornithine decarboxylase as a biomarker of aquatic toxicology

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dc.acquisition-srcDownloaded from-Aquatic Sciences and Fisheries Abstractsen_US
dc.call-noen_US
dc.contract-noen_US
dc.contributor.authorHaddox MKen_US
dc.contributor.authorSociety of Environmental Toxicology and Chemistry PUen_US
dc.contributor.otheren_US
dc.date.accessioned2010-02-15T17:14:07Z
dc.date.available2010-02-15T17:14:07Z
dc.date.issued1993 1993 Nov 14en_US
dc.degreeen_US
dc.description-Ecologicalen_US
dc.description-otheren_US
dc.description.abstractOrnithine decarboxylase (ODC) is the initial rate-limiting enzyme in the biosynthesis of polyamines, the organic cations of the cell. Expression of ODC is absolutely required for the growth of all cell types. Ornithine decarboxylase has the striking property among vertebrate enzymes in that under normal conditions it has a short intracellular half-life of 15-20 minutes and the level of expression is minimal. However, after exposure to a toxic or carcinogenic stimulus the amount of ODC activity in the tissue is markedly increased in parallel to the degree of toxicity, increasing from 25-1000-fold above the normal tissue. We have found that one of the molecular mechanisms which leads to this marked increase in ODC activity is an increase in the stability of the enzyme to intracellular proteolysis which is due to progressive increases in the phosphorylation state of the enzyme resulting in more acidic isoelectric forms of ODC. We have tested this phenomena as a potential biomarker of aquatic toxicity by analyzing livers of flounder from Galveston Bay which were normal versus cancerous. The activity of ODC in the cancerous flounder livers was 100-times greater than that present in the normal flounder livers. Our results suggest that analysis of the isoelectric status of ODC in livers from aquatic animals may provide a useful biomarker for the extent of aquatic toxicology. (DBO)en_US
dc.description.urihttp://gbic.tamug.edu/request.htmen_US
dc.historyen_US
dc.identifier.urihttp://hdl.handle.net/1969.3/23033
dc.latitudeen_US
dc.locationen_US
dc.longitudeen_US
dc.notes14. Annu. Meet. Society of Environmental Toxicology and Chemistry (SETAC) - Ecological Risk Assessment: Lessons Learned?, Houston (USA)Physical medium: Printed matter; Summary onlyEnglishBook Monograph; Conference; SummaryFreshwaterKE1993en_US
dc.placeen_US
dc.publisheren_US
dc.relation.ispartofseries50580.00en_US
dc.relation.urien_US
dc.scaleen_US
dc.series14th Annual Meeting (SETAC) - Ecological Risk Assessment: Lessons Learned? -- Abstract Book. vp. 1993en_US
dc.subjectAllellesen_US
dc.subjectanalysisen_US
dc.subjectAquatic Animalsen_US
dc.subjectASW,USA,Texas,Galveston Bayen_US
dc.subjectBiomarkersen_US
dc.subjectCarcinogensen_US
dc.subjectGalveston Bayen_US
dc.subjectgrowthen_US
dc.subjectLeaden_US
dc.subjectliveren_US
dc.subjectQ5 01502 Methods and instrumentsen_US
dc.subjectRisken_US
dc.subjectRisk assessmenten_US
dc.subjectTexasen_US
dc.subjectToxicityen_US
dc.subjectToxicologyen_US
dc.subjectUSAen_US
dc.titleIsoelectric shifts in ornithine decarboxylase as a biomarker of aquatic toxicologyen_US
dc.typeCONFen_US
dc.universityen_US
dc.vol-issue()en_US

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