Detection of Hepatitis A virus by hybridization with single-stranded RNA probes.

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dc.acquisition-srcen_US
dc.call-noen_US
dc.contract-noen_US
dc.contributor.authorJiang, X.en_US
dc.contributor.authorEstes, M.K.en_US
dc.contributor.authorMetcalf,T.G.en_US
dc.contributor.otherApplied and Environmental Microbiology.en_US
dc.date.accessioned2010-02-15T16:59:16Z
dc.date.available2010-02-15T16:59:16Z
dc.date.issued1987en_US
dc.degreeen_US
dc.descriptionp. 2487-2495.en_US
dc.description-otheren_US
dc.description.abstractAn improved method of dot-blot hybridization to detect hepatitis A virus (HAV) was developed with single-stranded RNA (ssRNA) probes. Radioactive and nonradioactive ssRNA probes were generated by in vitro transcription of HAV templates inserted into the plasmid pGEM-1. 32P-labeled ssRNA probes were at least eightfold more sensitive than the 32P-labeled double-stranded cDNA counterparts, whereas biotin-labeled ssRNA probes showed a sensitivity comparable with that of the 32P-labeled double-stranded cDNA counterparts. Hybridization of HAV with the ssRNA probes at high stringency revealed specific reactions with a high signal-to-noise ratio. The differential hybridization reactions seen with probes of positive and negative sense (compared with HAV genomic RNA) were used to detect HAV in clinical and field samples. A positive/negative ration was introduced as an indicator that permitted a semiquantitative expression of a positive HAV reaction. Good agreement of this indicator was observed with normal stool samples and with HAV-seeded samples. By using this system, HAV was detected in estuarine and freshwater samples collected from a sewage-polluted bayou in Houston and a saltwater tributary of Galveston Bay.en_US
dc.description.urihttp://gbic.tamug.edu/request.htmen_US
dc.geo-codeGalveston Bayen_US
dc.geo-codeHoustonen_US
dc.historyen_US
dc.identifier.urihttp://hdl.handle.net/1969.3/20715
dc.latitudeen_US
dc.locationNOT AVAILABLE IN-HOUSE.en_US
dc.longitudeen_US
dc.notesen_US
dc.placeen_US
dc.publisheren_US
dc.relation.ispartofseries291.00en_US
dc.relation.urien_US
dc.scaleen_US
dc.seriesen_US
dc.subjectpublic healthen_US
dc.subjecthepatitis Aen_US
dc.subjectwater qualityen_US
dc.subjectfresh wateren_US
dc.subjectestuariesen_US
dc.subjectsewageen_US
dc.subjectpublic healthen_US
dc.subjectdiseasesen_US
dc.subjectwater pollutionen_US
dc.titleDetection of Hepatitis A virus by hybridization with single-stranded RNA probes.en_US
dc.typeArticleen_US
dc.universityen_US
dc.vol-issue53(10)en_US

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