Ecology of Vibrio-Vulnificus in Galveston Bay Oysters, Suspended Particulate Matter, Sediment and Seawater - Detection by Monoclonal-Antibody Immunoassay Most Probable Number Procedures

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dc.acquisition-srcDownloaded from-Web of Scienceen_US
dc.call-noen_US
dc.contract-noen_US
dc.contributor.authorVanoy RWen_US
dc.contributor.authorTamplin MLen_US
dc.contributor.authorSchwarz JRen_US
dc.contributor.otherJournal of Industrial Microbiologyen_US
dc.date.accessioned2010-02-15T17:17:40Z
dc.date.available2010-02-15T17:17:40Z
dc.date.issued1992 Mayen_US
dc.degreeen_US
dc.description219-223en_US
dc.description-otheren_US
dc.description.abstractOysters, suspended particulate matter (SPM), sediment and seawater samples were collected from West Galveston Bay, Texas over a 16-month period and analyzed for the presence of Vibrio vulnificus, a naturally-occurring human marine pathogen. Detection and enumeration of V. vulnificus was performed using a species-specific monoclonal antibody (mAb FRBT37) in an enzyme immunoassay (EIA)-most probable number (MPN) procedure capable of detecting as few as 2000 target organisms. V. vulnificus was not detected in seawater, oyster or SPM samples during the cold weather months, but was detected at low levels in several sediment samples during this time period. Increased levels of the organism were first observed in early spring in the sediment, and then in SPM and oysters. The major increase in V. vulnificus occurred only after the seawater temperature had increased above 20-degrees-C and the winter-spring rainfall had lowered the salinity below 16 parts per thousand. The highest V. vulnificus levels at each site were associated with suspended particulate matter. These results are consistent with the hypothesis that (1) V. vulnificus over-winters in a floc zone present at the sediment-water interface, (2) is resuspended into the water column in early spring following changes in climatic conditions, (3) colonizes the surfaces of zooplankton which are also blooming during early spring and (4) are ingested by oysters during their normal feeding processen_US
dc.description.urihttp://gbic.tamug.edu/request.htmen_US
dc.historyen_US
dc.identifier.urihttp://hdl.handle.net/1969.3/23524
dc.latitudeen_US
dc.locationen_US
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dc.notesTimes Cited: 15ArticleEnglishCited References Count: 14JD731HOUNDMILLS, BASINGSTOKE, HAMPSHIRE, ENGLAND RG21 6XSBASINGSTOKEen_US
dc.placeen_US
dc.publisheren_US
dc.relation.ispartofseries51177.00en_US
dc.relation.urien_US
dc.scaleen_US
dc.seriesen_US
dc.subjectVIBRIO-VULNIFICUSen_US
dc.subjectoysteren_US
dc.subjectMONOCLONAL ANTIBODYen_US
dc.subjectMOST PROBABLE NUMBERen_US
dc.subjectENZYME IMMUNOASSAYen_US
dc.titleEcology of Vibrio-Vulnificus in Galveston Bay Oysters, Suspended Particulate Matter, Sediment and Seawater - Detection by Monoclonal-Antibody Immunoassay Most Probable Number Proceduresen_US
dc.typeJournalen_US
dc.universityen_US
dc.vol-issue9(3-4)en_US

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