Determining Genetic Diversity of the Seagrass Halodule Wrightii Using Random Amplified Polymorphic DNA (RAPD)

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dc.acquisition-srcGBEPen_US
dc.call-noQH 541.5 .E8 G32 T-5 c.1-3 GBAYen_US
dc.call-noREF QH 541.5 .E8 G32 T-5 c.1-4 GBAYen_US
dc.call-noARCHIVE QH 541.5 .E8 G32 T-5 c.1-3 GBAYen_US
dc.contract-noGBEP T-5en_US
dc.contributor.authorAngel, Rachelen_US
dc.contributor.otherProceedings: The State of the Bay Symposium V. January 31 - February 2, 2001en_US
dc.date.accessioned2010-02-15T17:36:57Z
dc.date.available2010-02-15T17:36:57Z
dc.date.issued2001en_US
dc.degreeen_US
dc.descriptionpg. 38en_US
dc.description-otheren_US
dc.description.abstractThe genetic diversity of two populations of the sea grass Halodule wrightii from Christmas Bay and a Flour Bluff cooling pond in Corpus Christi was determined using Random Amplified Polymorphic DNA (RAPD). H. wrightii from Florida Bay was used for comparison. H. wrightii with a high level of genetic diversity has the best chance of adapting to a recipient site after being transplanted to Galveston Bay, Texas, which has lost over 90% of its sea grass since 1956. Sea grass beds are economically important because they provide a nursery habitat for 28% of commercial fish species and 30% of blue crab and shrimp populations in Galveston Bay. Recent technological advances have made it possible to transplant large areas with sea grass using less time and money than previous methods. Isozyme analysis had shown that H. wrightii populations in Texas had low genetic diversity. RAPD is more sensitive to detecting genetic diversity than isozymes because it samples coding and noncoding sections of the genome. The number of loci amplified allows identification of individuals within a population. RAPD revealed a high level of polymorphism. An Analysis of Molecular Variance (AMOVA) showed that approximately 60% of genetic variation was found among populations indicating a heterogeneous species. UPGMA cluster analysis showed every individual except one clustered within its population. All individuals appeared to have unique genotypes. Corpus Christi clustered more closely to Florida Bay than to Christmas Bay. These results were not expected since Corpus Christi and Florida Bay are separated by 1600 km whereas Corpus Christi is only 400 km from Christmas Bay. The similar habitats of Corpus Christi and Florida Bay may be acting as a selection agent or mating time may differ between Christmas Bay and Corpus Christi thus preventing gene flow.en_US
dc.description.urien_US
dc.geo-codeHoustonen_US
dc.geo-codeGalvestonen_US
dc.geo-codeGalveston Bayen_US
dc.geo-codeChristmas Bayen_US
dc.geo-codeCorpus Christien_US
dc.geo-codeFlour Bluffen_US
dc.history10/25/04 easen_US
dc.identifier.urihttp://hdl.handle.net/1969.3/26165
dc.latitudeen_US
dc.locationGBIC Circulating Collection; GBIC Reference Collection; GBIC Archive Collectionen_US
dc.longitudeen_US
dc.notesThe author is representing the University of Houstonen_US
dc.placeAustin, TXen_US
dc.publisherTexas Natural Resource Conservation Commissionen_US
dc.relation.ispartofseries9076.00en_US
dc.relation.urihttp://gbic.tamug.edu/gbeppubs/T5/gbnep-T5.htmlen_US
dc.scaleen_US
dc.seriesen_US
dc.titleDetermining Genetic Diversity of the Seagrass Halodule Wrightii Using Random Amplified Polymorphic DNA (RAPD)en_US
dc.typeChapteren_US
dc.universityen_US
dc.vol-issueT-5en_US

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