Development of a method for concentration of rotavirus and its application to recovery of rotaviruses from estuarine waters

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dc.acquisition-srcen_US
dc.call-noAcc#259 - Box#2en_US
dc.contract-noen_US
dc.contributor.authorRao, V.C.en_US
dc.contributor.authorMetcalf, T.G.en_US
dc.contributor.authorMelnick, J.L.en_US
dc.contributor.otherApplied and Environmental Microbiologyen_US
dc.date.accessioned2010-02-15T16:57:20Z
dc.date.available2010-02-15T16:57:20Z
dc.date.issued1986en_US
dc.degreeen_US
dc.descriptionpgs. 484-488.en_US
dc.description-otheren_US
dc.description.abstractAs part of our studies on the ecology of human enteric viruses, an improved method for detection of rotaviruses in water was developed, and their presence in Galveston Bay was monitored. Samples (378 liters) of estuarine water adjusted to pH 3.5 and a final AlCl3 molarity of 0.001 were filtered through 25-cm pleated cartridge type filters (Filterite Corp., Timonium, Md.) of 3.0- and 0.45- micrometer porosity. Adsorbed virus was eluted with 1 liter of 10% tryptose phosphate broth, pH 9.5. Primary eluates were reconcentrated to a final volume of 10 to 20 ml by a simple and rapid magnetic iron oxide adsorption and elution procedure. Two percent casein at pH 8.5 effectively eluted rotavirus from iron oxide. A total of 21 of 72 samples of water, suspended solids, fluffy sediments, and compact sediments collected in different seasons in Galveston Bay yielded rotaviruses. Recovery of rotaviruses varied from 119 to 1,000 PFU/378 liters of water, 1,200 PFU/ 1,000 g of compact sediment, 800 to 3,800 PFU/378 liters of fluffy sediment, and 1,800 to 4,980 PFU from suspended solids derived from 378 liters of water based on immunofluorescent foci counts on cover slip cultures of fetal monkey kidney cells.en_US
dc.description.urihttp://gbic.tamug.edu/request.htmen_US
dc.geo-codeGalveston Bayen_US
dc.history5/22/06 easen_US
dc.identifier.urihttp://hdl.handle.net/1969.3/20385
dc.latitudeen_US
dc.locationGBIC Archives Collection; TAMUG Periodical Collectionen_US
dc.longitudeen_US
dc.notesen_US
dc.placeWashington, D.C.en_US
dc.publisherAmerican Society for Microbiologyen_US
dc.relation.ispartofseries259.00en_US
dc.relation.urien_US
dc.scaleen_US
dc.seriesen_US
dc.subjectvirusesen_US
dc.subjectestuariesen_US
dc.subjectecologyen_US
dc.subjectpublic healthen_US
dc.titleDevelopment of a method for concentration of rotavirus and its application to recovery of rotaviruses from estuarine watersen_US
dc.typeArticleen_US
dc.universityen_US
dc.vol-issuev. 52, no. 3en_US

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