Willett KLGardinali PRSericano JLWade TLSafe SHArchives of Environmental Contamination and Toxicology2010-02-152010-02-151997 Mayhttp://hdl.handle.net/1969.3/23490442-448The H4IIE rat hepatoma cell bioassay has been extensively used to assess the toxic equivalents (TEQs) of complex mixtures of halogenated aromatic hydrocarbons in environmental samples. However, there is often a discrepancy between bioassay induction results and toxic equivalents calculated from chemical analysis of samples; the former generally yield higher bioassay-TEQs. Polynuclear aromatic hydrocarbons (PAHs) are a class of chemicals which can significantly contribute to induction-TEQs, Benzo(a)pyrene (BAP), dibenz(a,h)anthracene (DBA), benz(a)anthracene (BA), benzo(k)fluoranthene (BkF), benzo(b)fluoranthene (BbF), chrysene (Chr), and indeno(1,2,3-c,d) pyrene (IdP) an carcinogenic PAHs found in environmental samples, including oysters collected from Galveston Bay. The induction potency of these PAHs relative to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) was determined individually in rat hepatoma H4IIE cells seeded in ti-well plates, and the induction-derived equivalency factors (EFs) relative to TCDD were 0.000354, 0.00203, 0.000025, 0.00478, 0.00253, 0.00020, 0.0011 for BAP, DBA, BA,BkF, BbF, Chr, and IdP, respectively Dilutions of a reconstituted PAH mixture containing 23 PAHs (744 to 4466 ng/g total PAHs) with constant percentages of BAP (4.5%), DBA (3.5%), BA (2.4%), BkF (3.7%), BbF (3.5%), Chr (4.7%), and IdP (4.2%) yielded bioassay-derived induction-EQs that ranged from 0.52 to 1.44 ng/g. Oysters exposed in the Laboratory to the same PAH mixture for 30 days differentially accumulated the PAHs with lime. Bioassay-EQs for these oyster extracts ranged from 0.94 to 5.79 ng/g, These results were similar to the chemically calculated EQs which varied from 0.81 to 3.13 ng/gTOXIC EQUIVALENCY FACTORSPOLYCHLORINATED-BIPHENYLSPLATE READERPOTENCYDIOXINCHROMATOGRAPHYEXTRACTSMIXTURESBINDINGJournal