Protocol for conducting sediment bioassays with materials potentially containing zebra mussels (Dreissena polymorpha)

The zebra mussel, Dreissena polymorpha, was introduced in United States waters accidentally in 1985 and has since become a major nuisance organism. Although many waters have become infested and distribution of the organism has become widespread, procedures are being developed to avoid release in waters that are not infested and to avoid additional release in those that are already infested. Techniques and procedures to avoid the unintentional release of the nonindigenous species during conduct of bioassays are discussed. This protocol contains methods for receiving and handling sediment and/or water samples used in bioassays that may potentially contain zebra mussels. Facility design, personnel requirements, effective treatments, and emergency guidelines are presented in the report.