Isolation and comparison of exopolymeric substances (EPS) from five dominant phytoplankton species in the Gulf of Mexico

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dc.contributor.authorKennady, Benjamin J
dc.contributor.authorChuang, Chia-Ying
dc.contributor.authorHo, Yi-fang
dc.contributor.authorSchwehr, Kathleen A.
dc.contributor.authorSantschi, Peter H.
dc.date.accessioned2012-06-01T19:43:30Z
dc.date.available2012-06-01T19:43:30Z
dc.date.issued2012-06-01
dc.descriptionFaculty advisor: Dr. Peter Santschien_US
dc.description.abstractInterest in EPS has recently been increasing as they have a great impact on many of geochemical processes, including prediction of POC flux, trace element, nanoparticle, and radionuclide scavenging, and tracing different water masses. EPS, released from marine micro-organisms are composed of polysaccharides and proteins, . Thus, there is a great need for these compounds to be characterized. Five phytoplankton species were chosen because of their dominance in the Gulf of Mexico. These species are Dunaliella tertiolecta, Thalassiosira pseudonana, Synechococcus elongates, Emiliania huxleyi, and Phaeodactylum tricornutum. The growth curves of these algae were monitored using a Spectrophotometer at 750 nm. By monitoring the growth curve, the transitional phase for each algae culture was estimated so that the EPS could be collected under similar conditions. EPS was harvested through a series of filtration processes between the sizes of 0.45 μm and 1 Kda. These non-attached EPS were tested using ATR-FTIR, Spectrophotometry, and CHN/S elemental analyzer. Results will be used to determine and compare the total polysaccharides, proteins, and elemental contents of each phytoplankton species, thus allowing for a better understanding of the total EPS composition in the Gulf of Mexico.en_US
dc.identifier.urihttp://hdl.handle.net/1969.3/28352
dc.language.isoen_USen_US
dc.relation.ispartofseriesTAMUG Student Research Symposium;8th Annual, 2012
dc.subjectexopolymeric substancesen_US
dc.subjectphytoplankton - chemical compositionen_US
dc.subjectDunaliella tertiolectaen_US
dc.subjectThalassiosira pseudonanaen_US
dc.subjectSynechococcus elongatesen_US
dc.subjectEmililania huxleyien_US
dc.subjectPhaeodactylum tricornutumen_US
dc.titleIsolation and comparison of exopolymeric substances (EPS) from five dominant phytoplankton species in the Gulf of Mexicoen_US
dc.typeImageen_US

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(2012) 8th Annual Student Research Symposium