Methods of Assessing the Toxicity of Sediment-associated Contaminants with Estuarine and Marine Amphipods

dc.call-noSPEC COLL GBAY ACC#11212
dc.creatorUnited States Environmental Protection Agency Office of Research and Development
dc.date.accessioned2011-03-10T01:56:09Z
dc.date.available2011-03-10T01:56:09Z
dc.date.issued1994-06
dc.description156 pages; available for download at the link below.en
dc.description.abstractA laboratory methods is described for determining the short-term toxicity of contaminated whole sediments using marine and estuarine amphipod crustaceans. Sediments may be collected from estuarine or marine environmentas or spiked with compounds in the laboratory. A test method is outlined that may be used with any of four amphipod species, including Ampelisca abdita, Eohaustorius estuarius, Leptocheirus plumulosus, and Rhepoxynius abronius. The toxicity test is conducted for 10 d in 1 L glass chambers containing 175 mL of sediment and 800 mL of overlying water. Overlying water is not renewed, and test organisms are not fed during the toxicity tests. Temperature and salinity of overlying water, and choice of negative control sediment, are species-specific. The choice of reference sediment may also be species-specific under certain applications. The endpoint in the toxicity test is survival, and reburial of surviving amphipods is an additional measurement that can be used as an endpoint. Procedures are described for use with sediments from oligohaline to fully marine environments.en
dc.identifier.otherAccession # 11212
dc.identifier.urihttp://hdl.handle.net/1969.3/27736
dc.language.isoen_USen
dc.locationGBIC Special Collection
dc.publisherUnited States Environmental Protection Agencyen
dc.relation.ispartofseriesEPA;600/R-94/025
dc.subjectsedimentsen
dc.subjecttoxicityen
dc.subjectbiological indicatorsen
dc.subjectenvironmental protectionen
dc.titleMethods of Assessing the Toxicity of Sediment-associated Contaminants with Estuarine and Marine Amphipodsen
dc.typeTechnical Reporten

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